Alignments corresponding to the study "Origin and evolution of key enzymes in the anammox pathway revisited".

This is a dataset containing the alignments generated (in FASTA format) via Diamond searches using proteins from “Candidatus Kuenenia stuttgartiensis” that have previously been identified in the anammoxosome and the anammox pathway were used as queries against a dataset constructed using all taxa under the class “Candidatus Brocadiia” in the NCBI taxonomy as of November 2022 (n=227), under the class Brocadiae in the GTDB taxonomy v.207 (n=38), and additional taxa covering all major clades of the PVC (Planctomycetota, Verrucomicrobiota and Chlamydiota) superphylum (n=58).

Multiple sequence alignments (MSAs) were calculated using MAFFT L-INS-I v.7.471 and trimmed with trimAl v1.4.1. These alignments were used as input to compute the phylogenetic trees using IQ-Tree v2.2.0 under the best-fitting model selected from LG and WAG.