Imaging · study · 2026
Spatial transcriptomic profiling of TOP2B-driven anthracycline-induced cardiotoxicity in mouse hearts
Listed in NCBI GEO
Anthracycline-induced cardiotoxicity (AIC) is a major dose-limiting toxicity in cancer patients, traditionally attributed to off-target inhibition of TOP2B in cardiomyocytes.
Description
Here, we performed spatial transcriptomic profiling of mouse hearts to investigate the molecular mechanisms underlying AIC and TOP2B-mediated cardiac dysfunction. Using a tamoxifen-inducible, cardiomyocyte-specific human TOP2B transgenic mouse model, we analyzed spatial gene expression changes associated with heart failure phenotypes.
Visium spatial RNA sequencing revealed dysregulation of cardiac contractile and metabolic pathways, including MYH6/MYH7 switching, NPPA upregulation, and UCP3 suppression. These data support a model in which TOP2B overexpression disrupts cardiomyocyte homeostasis and contributes directly to heart failure. This dataset provides a spatially resolved transcriptomic resource for studying TOP2B-driven cardiotoxicity and therapeutic intervention strategies.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319842 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319842 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1425298 ncbi.nlm.nih.gov/bioproject/PRJNA1425298 ↗
project · from NCBI GEO
- PubMed 42102394 pubmed.ncbi.nlm.nih.gov/42102394 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · Heart 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE319842 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |