Omics · study · 2026
Bulk RNA-seq in Tasor2 KO testis & Tex15 cKO germ cells
Listed in NCBI GEO
Chromatin-associated factors play critical roles in germ cell development.
Description
TEX15, a testis-specific protein, is essential for retrotransposon silencing and meiotic progression in male germ cells. Here we report that TEX15 plays a novel role in spermiogenesis.
Postnatal inactivation of TEX15 leads to teratozoospermia and male subfertility but not de-repression of retrotransposons. Mechanistically, TEX15 forms a complex with TASOR2 but not its paralogue TASOR in testis. Strikingly, TEX15 and HUSH2 components (TASOR2, MPP8, and PPHLN1) colocalize as nuclear foci at XY chromatin in round spermatids.
Read the rest (2 more)
The formation of TEX15 nuclear focus is dependent on TASOR2 but not vice versa, indicating that HUSH2 recruits TEX15 to the chromatin. TASOR2-deficient mice are viable but exhibit teratozoospermia. TASOR2 and MPP8 bind to muti-copy gene families in postmeiotic spermatids.
Importantly, multi-copy genes including X-linked H2al genes are upregulated in TEX15-deficient spermatids and TASOR2-deficient testis. Our results demonstrate that the HUSH2 complex targets and represses multi-copy gene families in spermiogenesis through TEX15, providing a mechanism for postmeiotic sex chromatin (PMSC) repression.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324264 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324264 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1434326 ncbi.nlm.nih.gov/bioproject/PRJNA1434326 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE324264 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |