Transcriptomic profiling of tumor-associated macrophages following CRISPR–Cas9 perturbation of myeloid lectins in a human breast cancer co-culture model

To investigate the contribution of individual myeloid lectin receptors to tumor-conditioned macrophage states, primary human monocytes from four healthy donors were subjected to CRISPR–Cas9 ribonucleoprotein (RNP)-mediated perturbation of CLEC4E, CLEC6A, CD209, SELL, or SIGLEC9 and subsequently co-cultured with BT474 breast cancer cells for 7 days.

Viable CD45-positive macrophages were isolated by fluorescence-activated cell sorting and analyzed by bulk RNA sequencing. The dataset enables paired, donor-matched analysis of target-specific and shared transcriptional responses to myeloid lectin perturbation.