Omics · study · 2026
Cryoprotectant formulation differentially shapes cardiac and immune transcriptomes in frozen storage
Listed in NCBI GEO
Cardiac tissue slices are used widely for biobanking and pharmacological testing, yet how freezing and cryoprotection reshape their molecular state remains poorly defined.
Description
We stored porcine cardiac tissue slices at -20 °C in a conventional DMSO-containing cryomedium or DMSO-free trehalose-based formulations, with or without supplementation, and profiled transcriptomes from day 1 to day 7 together with TUNEL apoptosis and CD68 immunophenotyping.
Transcriptional injury was formulation- and time-dependent, caused by freezing rather than 4 °C handling. The DMSO-containing cryomedium drove collapse of sarcomeric, calcium-handling and mitochondrial programmes, with key transcripts falling by -3 to -4.4 log2FC at day 7. DMSO-free storage preserved these programmes substantially better.
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The four supplemental protectants recovered myocardial metabolic programmes and attenuated necroptotic and ferroptotic signalling. Tissue-resident immunity was the most striking finding: complement, phagocytic and NET-related transcripts were preserved at day 1 in DMSO-containing storage, fell sharply in DMSO-free storage, and partially recovered by day 7, whereas DMSO-stored tissue showed progressive immune-associated activation.
Immunophenotyping revealed formulation-dependent CD68+ cell density that mirrored the transcriptomic immune signature.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344759 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344759 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1517364 ncbi.nlm.nih.gov/bioproject/PRJNA1517364 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Sus scrofa
- From keywords
- Life Sciences
- Inferred from text
- Food sciences 69% · Heart 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE344759 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].anzsrc:group:3006 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9823 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |