Omics · study · 2026
Single-nucleus transcriptomics of CLRN1 knockout rabbit retina reveals Müller glia-driven mechanisms of retinal degeneration
Listed in NCBI GEO
Single-nucleus RNA sequencing of wild-type and CLRN1 knockout rabbit retina at 10 months of age (pre-symptomatic timepoint).
Description
Three biological replicates per genotype were pooled and processed using 10x Genomics Chromium Single Cell 3' v3.1 platform. SNP-based demultiplexing with Vireo was used to assign nuclei to individual animals.
Analysis of 6,542 high-quality nuclei across 7 retinal cell types revealed that CLRN1 is exclusively expressed in Müller glia and identified a primary adherens junction defect (CTNNA2 downregulation) leading to secondary photoreceptor degeneration while inner retinal neurons mount robust compensatory responses.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320260 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320260 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1427218 ncbi.nlm.nih.gov/bioproject/PRJNA1427218 ↗
project · from NCBI GEO
- PubMed 42390169 pubmed.ncbi.nlm.nih.gov/42390169 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE320260 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |