Thermal Performance of The Tropical Marine cleaner shrimp (<i>Lysmata amboinensis</i>)

Shrimp were held at 25 °C before being exposed to either increasing or decreasing temperatures, with ventilation recorded throughout the thermal ramp. These data were used to estimate CT_max, CT_min, and T_opt. Metabolic responses were assessed by measuring tissue concentrations of ATP, glucose, and L-lactate at 10, 25, 29, 31, and 34 °C using spectrophotometric enzymatic assays.</p><p dir="ltr"><b>Data included</b></p><p dir="ltr">Five comma-separated values (CSV) files are provided:</p><ul><li><b>shrimpscathog.csv-</b> scaphognathite beat frequency (ventilation) data collected during the warming and cooling thermal performance experiments.

These data were used to construct thermal performance curves and estimate CT_max, CT_min, and T_opt.</li><li><b>controlalldata.csv</b> - data collected from control shrimp maintained at 25 °C during the corresponding thermal performance experiments.</li><li><b>ATPdata.csv</b> - ATP concentration data from shrimp sampled at the experimental temperatures.</li><li><b>Glucosedata.csv</b> - glucose concentration data from shrimp sampled at the experimental temperatures.</li><li><b>lactatedata.csv</b> - L-lactate concentration data from shrimp sampled at the experimental temperatures.</li></ul><p dir="ltr">The data were processed in Microsoft Excel and analysed in RStudio (v2023.12.0).

Statistical analyses included linear mixed-effects models for ventilation data and one-way ANOVA with post hoc comparisons for metabolite data.</p><p dir="ltr">The study found a temperature of optimum ventilatory performance of 30.25 °C, with a CTmax of 35.25 °C and CTmin of 7.5 °C. These data provide a characterisation of both upper and lower thermal performance and tolerance in <i>L. amboinensis</i>.</p><p dir="ltr">This dataset is associated with an unpublished manuscript currently submitted.

Publication details and a link to the associated article will be added when available.</p><p dir="ltr"><b>Ethical and legal considerations:</b> All experimental procedures were approved by the University of Plymouth Ethics Board. As decapod crustaceans are not currently protected under the UK Animals (Scientific Procedures) Act 1986, this study did not require Home Office Licensing under ASPA. However, all procedures were conducted in accordance with the ARRIVE 2.0 guidelines and followed institutional animal welfare best practices, reflecting the welfare status afforded to decapod crustacean under the UK Animal Welfare (Sentience) ACT 2022.

Animals were humanely terminated immediately upon reaching critical thermal endpoint by rapid separation of the cephalothorax, using a chilled scalpel blade, severing the central nervous cord and cephalic ganglia. </p>