Data · study · 2026
Single intranasal dose of L-MYC–immortalized human neural stem cells enhances neuroprotection and tissue repair after controlled cortical impact in male rats
Listed in NCBI GEO
Traumatic brain injury (TBI) initiates cellular and molecular cascades that impair neurogenesis and promote chronic neuroinflammation, ultimately contributing to cognitive and functional decline.
Description
We previously demonstrated that L-myc–expressing human neural stem (LMNSC01) cells migrate to sites of injury and modulate biomarkers associated with neuroprotection and repair after repeated intranasal (IN) administration. Here, we tested whether a single bulk IN dose of LMNSC01 is sufficient to induce neuroprotective changes in gene and protein expression following injury.
Immunocompetent male rats underwent controlled cortical impact (CCI) or sham surgery, followed by a single bulk IN dose of LMNSC01 or vehicle on postoperative day 7. Transcriptomic profiling of brain tissue from injured and LMNSC01-treated rats revealed upregulation of immune modulation and tissue repair genes (Il22ra1, Il1rn, Gfi1, Ddx60) and downregulation of genes involved in microglial activation and cytokine signaling (Xcr1, Ccl21a, Ccl9) compared to controls.
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Proteomic analysis confirmed reduced levels of inflammatory cytokines and endothelial activation markers (ICAM-1, PDGF-AA, TCK-1). Multiplex immunohistochemistry showed LMNSC01-mediated changes in microglial activation, macrophage infiltration, and vascular remodeling. These findings highlight the potential of single IN LMNSC01 dose to reprogram neuroinflammatory and tissue remodeling networks and offer a clinically translatable strategy to improve long-term outcomes after TBI.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322819 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322819 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1431911 ncbi.nlm.nih.gov/bioproject/PRJNA1431911 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by array · Mus musculus · Rattus norvegicus
- From keywords
- Life Sciences
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE322819 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-array | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |