Transcriptional landscape of NFIX defines developmental and regenerative programs in skeletal muscle

This remarkable tissue exhibits extraordinary regenerative potential, sustained by muscle stem cells, termed satellite cells. Among key players governing muscular homeostasis, Nuclear Factor I X (NFIX) is a transcription factor that plays fundamental roles during myogenesis as well as in adult muscle regeneration.

However, molecular mechanisms involving NFIX expression are nowadays mostly undiscovered. Here, combining multi-omic data analysis and in-vitro validation, we resolved the Nfix transcriptional landscape in skeletal muscle tissue. Our results indicate that epigenetic alterations and intragenic enhancers strongly control Nfix promoters’ activity, that together with alternative splicing processes affects Nfix isoforms’ expression.