Supplementary file 1_Myeloid EGR1 contributes to Th17/Treg imbalance during acute liver allograft rejection in a rat model.docx

Early growth response 1 (EGR1) mediates stress and inflammation, yet its cellular distribution and potential involvement in adaptive alloimmunity during distinct post-transplant stages remain unclear.</p>Methods<p>A rat orthotopic liver transplantation model was used to evaluate graft injury and EGR1 spatiotemporal expression during IRI (6–24 h) and AR (5–10 d).

Public scRNA-seq data and virtual knockout analysis were used to assess the cellular distribution and potential regulatory role of Egr1. EGR1-overexpressing bone marrow-derived dendritic cells (BMDCs) were co-cultured with CD4⁺ T-cells to assess cytokine secretion, proliferation, and shifted Th17/Treg differentiation.</p>Results<p>Both syngeneic and allogeneic grafts exhibited early IRI. By days 5–10, allografts developed AR with elevated liver enzymes and Banff scores.