Omics · study · 2026
Local tolerance and efficient innate immune activation of primary human respiratory cells by the recombinant flagellin FLAMOD
Listed in NCBI GEO
Antibiotic-resistant respiratory infections can lead to treatment failure, highlighting the need for alternative strategies.
Description
FLAMOD, a recombinant flagellin and Toll-like receptor 5 agonist, stimulates innate immunity when delivered intranasally. In mouse models, FLAMOD is effective against bacterial pneumonia.
The protective efficacy was associated with activation of airway epithelial cells. This study aimed to assess the tolerance of human primary respiratory epithelium to FLAMOD administered apically as liquid droplets or by nebulization, to measure the FLAMOD-mediated innate immune response, and to analyze the pharmacokinetics of FLAMOD. We used epithelia reconstituted from human nasal and bronchial (MucilAir™), small airways (SmallAir™) and alveolar (AlveolAir™) primary epithelial cells, cultured at the air-liquid interface.
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We report that daily administration of escalating doses of FLAMOD for 5 days was well tolerated in the various epithelium as epithelial integrity, cilia motion and viability were not affected. FLAMOD was rapidly degraded and leakage into the basal compartment was not detected. An immunomodulatory effect was identified in each epithelium, and found to be FLAMOD dose-dependent with an effective dose in the picomolar range.
Similar safety profile and immune responses were obtained with airway epithelium from cystic fibrosis and chronic obstructive pulmonary disease patients. In conclusion, this study supports the development of the use of TLR5 agonist for respiratory delivery to stimulate innate defenses against infections of vulnerable patients.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313945 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313945 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1387412 ncbi.nlm.nih.gov/bioproject/PRJNA1387412 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313945 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |