DCLK1 regulates stemness and IL-6/STAT3–dependent metastatic niche formation in chemoresistant ovarian cancer

Targeted mRNA expression profiling was performed using nCounter® Tumor Signaling 360™ Panel (NanoString Technologies) composed of 780 genes (including internal reference genes) involved in cellular energetics, sustained proliferation, evasion of growth suppression, genomic instability, resistance to cell death, epithelial to mesenchymal transition (EMT), and metastasis. Normalization of housekeeping genes for the quantification of gene expression levels, normalization of the positive control for background correction, and data analysis were performed using the ROSALIND Software version 3.16 (Rosalind) for quality control, positive-control normalization, and housekeeping gene normalization.