Data · dataset · 2022
Efficient healing of large osseous segmental defects using optimized chemically modified messenger RNA encoding BMP-2
Listed in IISH Dataverse
Large segmental osseous defects heal poorly, creating major medical, social and economic issues.
Description
Although several clinical options for healing such defects exist, all have major disadvantages. We describe a novel approach to bone regeneration using chemically modified messenger RNA (cmRNA).
An optimized cmRNA encoding bone morphogenetic protein 2 (BMP-2) was delivered to critical size, mid-diaphyseal, femoral osteotomies in rats. The cmRNA remained orthotopically localized and generated BMP locally for several days. Defects healed at doses > 25 µg BMP-2 cmRNA.
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By four weeks, all animals treated with 50 µg BMP-2 cmRNA had bridged bone defects without forming the massive callus seen with rhBMP-2. Transcriptomic analysis and immunohistochemistry confirmed that cmRNA regenerated bone via endochondral ossification, whereas rhBMP-2 drove intramembranous bone formation. The osteogenic properties of the optimized cmRNA were superior to those of rhBMP-2 and provide the basis of an innovative, safe and highly translatable technology for healing osseous defects.
Links
Where it is published
- Dataverse dataset page dataverse.nl/dataset.xhtml?persistentId=doi%3A10.34894%2FXUOHLG ↗
landing page · from datasets iisg amsterdam
- DOI doi.org/10.34894/xuohlg ↗
DOI / persistent id · from datasets iisg amsterdam
Catalogue records · 1
- Dataverse API dataverse.nl/api/datasets/:persistentId/?persistentId=doi%3A10.34894%2FXUOH… ↗
metadata API · from datasets iisg amsterdam
Topics
- Stated by source
- Medicine, Health and Life Sciences
- From keywords
- Life Sciences · Medicine & Health
- Inferred from text
- Medical biotechnology 70%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| IISH Dataverse | doi:10.34894/XUOHLG | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3206 | enrichment · datasets iisg amsterdam | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].dataverse_subject:medicine-health-and-life-sciences | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /subjects |
| created_date | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | |
| description | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /description |
| publication_date | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | |
| title | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | /name |
| updated_date | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 | |
| version_label | source · datasets iisg amsterdam | connector:datasets_iisg_amsterdam@1.0.0 |