Omics · study · 2026
APOE genotype modifies the gene signature of microglial and astrocytic responses to aging
Listed in NCBI GEO
To determine how APOE genotypes impact the gene signature of microglia and astrocytes during aging at single cell level, we isolated brain cells from young (3 month of age) and aged (24 month of age) APOE2 and APOE3 and APOE4 mice, and conducted single cell RNA sequencing (scRNAseq) (4 mice/age/genotype).
Description
We annotated all the cells based on their gene markers and then focused on microglia and astrocytes for further downstream analysis.
We functionally annotated each subcluster with microglia and astrocytes, based on the expression pattern of the widely accepted typical maker genes from multiple publications. Further, we identified the differentially expressed genes (DEG) in the comparison between young and aged mice for each subcluster in each APOE genotype and characterized the pathways related to these DEGs. Taken together, our dataset provided comprehensive resources to understand the APOE-regulated microglial and astrocytic responses to aging.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE285nnn/GSE285471 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE285471 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1203890 ncbi.nlm.nih.gov/bioproject/PRJNA1203890 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Genetics 68% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE285471 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |