Heterogenous microglial reactivity contrasts with stable vascular transcriptional programs in mouse models of Alzheimer’s, CADASIL, and Traumatic Brain Injury (ArcSwe data)

To address this, we established a transcriptomic repository of major vascular cell types and microglia to compare the transcriptomic response in mouse models of three human brain disorders linked to neuroinflammation and associated vascular reactivity: Alzheimer’s disease (AD), traumatic brain injury (TBI), and cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

Single-cell analysis of >250,000 cells at different disease stages led to identification of two previously unknown vascular cell subtypes, expanded the endothelial zonation spectrum and allowed for a detailed analysis of the molecular responses in the vascular cells and microglia. Surprisingly, all vascular cell types remained transcriptomically normal across the three conditions, while microglia exhibited significant, disease-specific transcriptional changes.