Omics · study · 2026
Muco-Obstructive Lung Disease Exhibits Distinct Hypoxic and Inflammatory Airway Epithelial Gene Pathways
Listed in NCBI GEO
Intraluminal obstruction produces chronic airway epithelial hypoxia.
Description
We investigated how chronic airway epithelial hypoxia and inflammatory stimuli (supernatatants of mucopurulent materials: SMM) interact in human bronchial epithelial (HBE) cultures and resected bronchiectatic lungs to produce the diseased epithelial environment characteristic of muco-obstructive diseases. Results: Hypoxia and supernatants of mucopurulent materials (SMM) regulated largely separate transcriptional programmes regardless of treatment order.
PTGS2 and CXCL8 represented a small number of genes additively upregulated by both hypoxia and SMM. HIF1A knockdown and EPAS1 knockdown produced distinct, often opposing, effects on hypoxia-responsive pathways. HIF1A dominated upregulation of glycolytic pathway signalling.
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EPAS1 regulated genes that increase mucus concentration under hypoxia (e.g., SCNN1G). Conclusion: Chronic hypoxia and inflammation regulated distinct genes/pathways that combine to produce complex diseased environments in muco-obstructed airways. HIF1α and EPAS1 regulated distinct hypoxia-induced transcriptional responses.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314018 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314018 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1389472 ncbi.nlm.nih.gov/bioproject/PRJNA1389472 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE314018 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |