Effect of keratinocyte-intrinsic VISTA deletion on UV-induced type I interferon and inflammatory gene expression in mouse epidermis [bulk RNA-seq]

Here we report that the immune checkpoint VISTA (encoded by Vsir) is expressed in epidermal keratinocytes and acts cell-intrinsically to suppress cGAS–STING–dependent IFN-I induction following ultraviolet (UV) exposure.

Conditional deletion of Vsir in keratinocytes (K14Cre-Vsir^fl/fl^) results in markedly elevated expression of interferon-stimulated genes (ISGs), chemokines, and antigen presentation machinery at baseline and is further amplified by acute UVB irradiation. Co-deletion of STING (Sting1) abrogates this hyperinflammatory transcriptional program, placing VISTA upstream of the cGAS–STING–IFN-I axis. Mechanistically, our data support a model in which VISTA restrains EGFR/LRIG1/NUMB-dependent stabilization of phosphorylated (pY245) STING, thereby tuning the magnitude of UV-induced IFN-I output.