Excel · study · 2026
Transcriptomic profiling of hemin-treated bone marrow-derived macrophages in a TGF-β1-induced macrophage–renal tubular epithelial cell co-culture model
Listed in NCBI GEO
Kidney fibrosis is characterized by persistent activation of renal tubular epithelial cells and extensive remodeling of the immune microenvironment.
Description
Macrophages are highly plastic cells that respond to metabolic and inflammatory signals and may regulate the progression of renal fibrosis. This study investigated the transcriptional changes induced by hemin in bone marrow-derived macrophages in a macrophage–renal proximal tubular epithelial cell co-culture system.
Mouse bone marrow-derived macrophages were co-cultured with primary renal proximal tubular epithelial cells using a Transwell system. Renal tubular epithelial cells were exposed to transforming growth factor beta 1 (TGF-β1) to establish a profibrotic microenvironment, with or without hemin treatment. After treatment, macrophages were collected for bulk RNA sequencing.
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Transcriptomic analysis showed that hemin altered macrophage pathways associated with heme and iron metabolism, antioxidant responses, glutathione metabolism, inflammatory signaling, extracellular matrix regulation, and cellular metabolism. These data provide insight into the macrophage transcriptional reprogramming associated with the protective effects of hemin against renal fibrosis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343718 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343718 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1512445 ncbi.nlm.nih.gov/bioproject/PRJNA1512445 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 68% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE343718 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |