Omics · study · 2026
Transcriptome and behavioral assessment in larval zebrafish (Danio rerio) following exposure to perfluorononanoic acid (PFNA)
Listed in NCBI GEO
Per- and polyfluoroalkyl substances (PFAS) are environmentally persistent chemicals widely detected in aquatic systems and drinking water.
Description
Perfluorononanoic acid (PFNA), a long-chain PFAS, has been reported globally in environmental matrices and fish tis-sues. Although PFNA has been linked to developmental, metabolic, and neurological toxicity, its effects on lipid-related pathways and neurotoxicity remain poorly charac-terized.
This study evaluated the developmental and neurotoxic effects of PFNA ex-posure in zebrafish embryos and larvae following a 7-day exposure to environmentally relevant PFNA concentrations. PFNA exposure did not significantly affect survival or deformity rates. Apoptosis was significantly increased in larvae exposed to 1 µg/L PFNA compared to controls, whereas reactive oxygen species were unaffected.
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Each tested concentration (0.1 µg/L and 10 µg/L) showed a different effect on the zebrafish transcriptome. Low-dose PFNA exposure primarily affected lipid transport, choles-terol metabolism, sphingolipid signaling, and neurodegeneration-related pathways, while high-dose PFNA altered synaptic signaling, axon guidance, and thyroid hormone synthesis. Hypoactivity was observed in the movement of larval zebrafish based on a visual motor response test.
Taken together, PFNA exposure leads to molecular changes related to neurotoxicity and lipid metabolism in zebrafish, which may con-tribute to adverse neurodevelopmental outcomes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324353 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324353 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1434461 ncbi.nlm.nih.gov/bioproject/PRJNA1434461 ↗
project · from NCBI GEO
- PubMed 42195015 pubmed.ncbi.nlm.nih.gov/42195015 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Danio rerio · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE324353 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:7955 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |