Omics · study · 2026
Transcriptomic Profiling of Mitochondrial RNA in HepG2 Cells Exposed to GenX, a Short-Chain PFAS
Listed in NCBI GEO
This study investigated the transcriptomic effects of GenX on the mitochondria of human liver HepG2 cells.
Description
Cells were exposed to GenX at 10 µM and 100 µM concentrations for 48 hours. Mitochondria were isolated and mitochondrial RNA was extracted using TRIzol.
High-quality RNA samples were sequenced using ribosomal-RNA-depleted libraries on the Illumina NovaSeq platform. Per and polyfluoroalkyl substances (PFAS) such as hexafluoropropylene oxide dimer acid (GenX) are emerging environmental contaminants with increasing concern regarding their potential mitochondrial toxicity. This BioProject investigated the effects of GenX exposure on mitochondrial gene expression in human hepatic HepG2 cells.
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Cells were treated with GenX at 10 µM and 100 µM concentrations for 48 hours, followed by mitochondrial isolation and RNA sequencing of mitochondrial transcripts. Mitochondrial transcriptomic analysis revealed regulation of genes involved in oxidative phosphorylation, redox homeostasis, and apoptotic signaling. This dataset provides a resource for understanding PFAS induced mitochondrial dysfunction and hepatotoxicity mechanisms.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342364 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342364 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1507446 ncbi.nlm.nih.gov/bioproject/PRJNA1507446 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Bioinformatics and computational biology 70% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE342364 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3102 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |