Omics · study · 2026
Derivation of Dome-Shaped Trophoblast Stem Cells with Morphology Distinct from Classic Trophoblast Stem Cells in Mice
Listed in NCBI GEO
The placenta serves as a critical interface between the mother and fetus, with placental dysfunction being a major contributor to various pregnancy complications.
Description
Successful placental formation relies on the robust expansion of trophoblast stem cells, followed by precisely regulated lineage specification. Trophoblast stem cells (TSCs) have emerged as a valuable model for investigating placentation and related disorders.
However, despite their ability to be maintained over multiple passages without losing stemness, conventional TSCs are characterized by inherent heterogeneity and a spontaneous tendency to differentiate autonomously. In this study, we established a novel type of mouse trophoblast stem cell. These cells form dome-shaped colonies, morphologically distinct from tight epithelial classic TSC colonies with a raised edge, and are therefore termed dome-shaped TSCs (dTSCs).
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The established dTSCs maintain the dome-shaped morphology, express canonical TSC markers, exhibit reduced cellular heterogeneity, display a relatively low propensity for spontaneous differentiation under stem cell maintenance conditions, retain multipotency for differentiating into specialized trophoblast lineages, and are capable of integrating into E12.5 placentas in chimeric assays. Meanwhile, dTSCs are amenable to gene manipulations, providing a practical means for studying functional genomics in the trophoblast lineages.
Collectively, these findings establish dTSCs as a promising and refined model for studying trophoblast development.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315936 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315936 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1399983 ncbi.nlm.nih.gov/bioproject/PRJNA1399983 ↗
project · from NCBI GEO
- PubMed 42757427 pubmed.ncbi.nlm.nih.gov/42757427 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE315936 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |