BRG1-dependent regulation of Tceal7 promotes myoblast differentiation

The data suggest that both Brg1-driven chromatin remodeling and Cn signaling converge on Tceal7 regulation. Functionally, Tceal7 knockdown altered cell proliferation and disrupted myoblast differentiation, at least in part due to reduced expression of Myogenin, which encodes a transcription factor that is an essential differentiation determinant. RNA-seq analysis revealed broad dysregulation of myogenic, metabolic, and cell-cycle gene programs in Tceal7-deficient cells, including changes in cyclin-dependent kinase-regulated pathways consistent with prior reports linking TCEAL7 to cell-cycle control.

Together, these findings identify TCEAL7 as a necessary component of the myogenic regulatory network whose expression is controlled by Brg1-dependent chromatin remodeling and Cn activity.