Sex specific effects of adoptive Tregs transfer on the brain and periphery in maternal immune activation offspring rescuing immune dysregulation

In addition, females were weighed after separation from males, and again 9-12 days later to verify pregnancy. MIA was induced in pregnant dams with polyinosinic-polycytidylic acid (poly I:C) as previously described [36].

Briefly, at gestational day 12.5, pregnant dams were injected intraperitoneally with a single dose (20 mg/kg body weight) of poly I:C (Sigma Aldrich; St. Louis, MO) or saline solution. When offspring reached 8 weeks of age, mice were injected via the tail vein with 50 µL of sterile PBS or 50 µL of sterile PBS containing 2 x 106 Tregs.