Stag2-mediated chromatin dynamics regulates antibody class switch recombination [3C-HTGTS]

However, the detailed regulatory mechanism of cohesin in CSR is largely lacking. Here, we revealed that Stag2 is a specific cohesin regulator playing critical roles in promoting CSR.

Stag2-deficiency, not Stag1-deficiency, significantly decreases the interaction of acceptor CH with CSR center, leading to decreased transcription of acceptor CH and impaired CSR. Notably, Stag2 could compensate for Stag1 binding within Igh upon Stag1-deficiency, while Stag2-deficiency significantly decreases Stag1 binding within Igh. Interestingly, Stag2 expression is higher than Stag1 during germinal center (GC) B cell development and Stag2 expression has a high correlation with CSR level in GC B cells upon vaccination and SARS-Cov-2 infection.