Omics · study · 2026
RNA-seq analysis of BMDMs from WT and Lcn10-KO mice
Listed in NCBI GEO
Bone marrow-derived macrophages (BMDMs) were isolated from wild-type (WT) and Lipocalin 10 knockout (Lcn10-KO) mice and subjected to RNA-seq to investigate the transcriptional alterations associated with Lcn10 deficiency in macrophages.
Description
Total RNA was extracted from cultured BMDMs, and sequencing libraries were generated and sequenced on an Illumina NextSeq 500 platform using a single-end 85 base pair sequencing strategy.
The resulting transcriptomic data were subsequently used for differential gene expression analysis and downstream pathway enrichment analyses to identify biological processes and signaling pathways potentially regulated by Lcn10 in macrophages.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330517 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330517 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1464545 ncbi.nlm.nih.gov/bioproject/PRJNA1464545 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE330517 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |