Omics · study · 2026
Transcriptomic profiling of LPS-stimulated RAW 264.7 macrophages with or without osteopontin treatment
Listed in NCBI GEO
Osteopontin (OPN/SPP1) has been implicated in the persistence of inflammatory macrophages in osteoarthritis synovium.
Description
This study examined transcriptomic changes induced by OPN in LPS-stimulated RAW 264.7 mouse macrophages. Cells were treated with LPS alone or pretreated with recombinant mouse OPN before LPS stimulation, and RNA-seq was performed using three independent biological replicates per condition.
The resulting profiles were used to evaluate OPN-associated transcriptional programs in inflammatory macrophages, including pathways related to cell proliferation and apoptosis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345213 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345213 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1519173 ncbi.nlm.nih.gov/bioproject/PRJNA1519173 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE345213 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |