Omics · study · 2026
Subepithelial myofibroblasts enhance stem cell regeneration after intestinal epithelial damage [RNA-Seq]
Listed in NCBI GEO
The intestinal epithelium relies on the surrounding niche environment to maintain homeostasis, but how the niche supports intestinal stem cell regeneration is unclear.
Description
We analyzed a number of distinct mesenchymal populations in the intestinal stem cell niche following two models of murine intestinal injury, including whole body irradiation and diphtheria-toxin injected Lgr5Dtr mice. We isolated Pdgfra fibroblasts from the murine transgenic PDGFRAH2BEGFP (GFP-hi and GFP-lo) and PdgfraCreERT2;Rosa26tDtomato (tDtomato+) mice, smooth muscle from Myh11CreERT2;PdgfraH2BeGFP;Rosa26tDtomato (GFP-tDtomato+), and blood and lympathic endothelium via antibody for CD31+LYVE1- and CD31+LYVE1+, respectively.
We found distinct molecular signatures of these cell types following stem cell loss and focused our analyses on known trophic factors of the overlaying intestinal epithelium.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322564 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322564 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1430554 ncbi.nlm.nih.gov/bioproject/PRJNA1430554 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE322564 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |