Omics · study · 2026
Bulk RNA-sequencing after estrogen-related receptorr knockdown (ESRRA) knockdown in human GIST-T1 cell line
Listed in NCBI GEO
Gastrointestinal stromal tumor (GIST) is the most common sarcoma and primarily driven by a gain-of-function mutation in either the KIT or PDGFRA receptor tyrosine kinase.
Description
Estrogen-related receptor alpha (ERRα, encoded by ESRRA) is an orphan nuclear receptor whose role in GIST is unknown. We found that ERRα was highly expressed in human GIST cell lines and human GIST surgical specimens.
Pharmacologic inhibition of ERRα using XCT90 or siRNA-mediated knockdown reduced viability, colony formation, and migration of human GIST cell lines.To further examine ERRα's role in regulating gene transcription in human GIST, we performed RNA-sequencing after siRNA-mediated knockdown of ERRα to identify changes in mRNA expression. We also sequenced T1 cells after imatinib treatment to compare the two treatments with each other in addition to the non-target controls.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330412 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330412 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1463807 ncbi.nlm.nih.gov/bioproject/PRJNA1463807 ↗
project · from NCBI GEO
- PubMed 42396531 pubmed.ncbi.nlm.nih.gov/42396531 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE330412 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |