Data Sheet 1_Identification of heterogeneous subtypes of human dendritic cells from the lymph nodes of patients with gastric cancer.pdf

Transcriptome and epitope analyses identified 26 clusters with distinct surface markers and gene expression profiles. DC lineages included subtypes distinct from the typical cDC1, cDC2, and pDC types. A proportion of CD1c-expressing cDC2 lineage cells expressed surface features characteristic of cDC1 and pDC, and CD1c expression was observed in cell types other than DC lineages.

Differences in DC cluster frequencies across DC lineages were not detected as significant across clinical stages and molecular subtypes of GC. The mRNA expression pattern in the CD141⁺ cDC1 lineage distinguished it from the CD1c⁺ cDC2 lineage and resolved different clusters with transitional states within both lineages. Transcripts related to DC maturation and migration were detected in a CD137-DC subtype, which highly expressed HLA-DR and CD137.</p>Conclusion<p>Cluster analysis of DC-enriched cells in LNs from our integrated dataset revealed subtypes of the cDC1, cDC2, and pDC lineages.

We identified distinctive DC subtypes in the transitional state bridging differentiation between cDC1 and cDC2. CD137-DC may be an independent DC subset with mature and migratory features.</p>