Omics · study · 2026
Histone H3 availability is more important for development than H3.2 versus H3.3 subtype identity
Listed in NCBI GEO
The distinct contributions of replication-dependent (RD) and replication-independent (RI) histones to development and genome function remain unclear.
Description
We investigate how the RI expression and distinct protein identities of the histone H3.2 and H3.3 subtypes influence Drosophila development and gene regulation. Replacing RI H3.3 genes with RD H3.2 revealed that RI H3.3 is essential for fertility, adult locomotor behavior, and normal lifespan.
However, development to adulthood does not depend on which RI H3 subtype is expressed. RI H3 is dispensable for establishing or maintaining global chromatin accessibility or gene expression in the adult brain. H3.2 expression becomes essential in post-mitotic cells lacking RI H3.3, and the HIRA histone chaperone complex in critical for preserving genome function when RI H3.3 is deleted.
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We conclude that an available pool of H3 is more critical than the specific identity of H3 in the pool.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317848 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317848 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1415669 ncbi.nlm.nih.gov/bioproject/PRJNA1415669 ↗
project · from NCBI GEO
Topics
- Stated by source
- Drosophila melanogaster · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE317848 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:7227 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |