Excel · study · 2026
RNA-seq profiling of human embryoic stem cell–derived ventral midbrain dopaminergic lineage cells across four differentiation stages
Listed in NCBI GEO
We performed bulk RNA sequencing to characterize temporal transcriptional dynamics during developmentally guided differentiation of human embryoic stem cells (hESCs) into ventral midbrain dopaminergic (vmDA) lineage cells.
Description
Samples were collected at four defined stages: undifferentiated hESCs (DIV 0), early patterned progenitors (DIV 9), late vmDA progenitors (DIV 16), and maturing vmDA neurons (DIV 35). Transcriptome analysis identified stage-specific gene expression programs corresponding to loss of pluripotency, activation of ventral midbrain patterning networks, and induction of neuronal maturation and synaptic function pathways.
This dataset provides a temporal molecular framework for vmDA lineage specification and maturation under an optimized differentiation protocol.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319963 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319963 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1425799 ncbi.nlm.nih.gov/bioproject/PRJNA1425799 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE319963 | 7 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |