TGIRT-seq-based quantification of snoRNAs across 5 healthy mouse organs

TGIRT-seq relies on a thermostable group II intron reverse transcriptase with high fidelity, processivity, and structure-tolerant activity, making it particularly well suited to accurately capture and quantify these RNA species.

This dataset presents a TGIRT-seq atlas generated from ribodepleted total RNA of five healthy mouse organs (bone, colon, gonad, liver and lung), enabling accurate snoRNA quantification alongside mRNAs, lncRNAs and other structured non-coding RNAs (e.g. tRNAs), using a genome annotation supplemented with an updated murine snoRNA annotation and tRNA gene annotations from GtRNAdb.