Single-nucleus transcriptomic sequencing of noise-induced hearing loss and regeneration in Xenopus laevis

Five experimental groups were set: a control group and four noise-exposed groups at days 1 (D1), 11 (D11), 21 (D21), and 37 (D37) post noise exposure. Single-nucleus RNA sequencing (snRNA-seq) was performed on inner ear tissues of all groups using the Illumina NovaSeq X Plus platform with paired-end sequencing mode.

We systematically characterized the dynamic changes of inner ear cell types, gene expression profiles, and pseudotime developmental trajectories at different injury and regeneration stages, providing a high-resolution transcriptomic data resource for revealing the molecular mechanisms underlying auditory injury repair and inner ear regeneration.