BMP-7 mRNA Delivered by Fibrin–CaP Scaffolds Activates Osteogenic Programs In Vivo as evidenced by transcriptomic and proteomic analyses

However, their clinical performance is limited by high costs, the need for supraphysiological doses, and adverse side effects. Here, we describe a chemically modified mRNA (cmRNA) encoding BMP-7 that promotes osteogenesis and functional ossification.

The BMP-7 cmRNA is delivered using optimized lipid vectors and a composite fibrin–calcium phosphate scaffold. Among several lipids evaluated, two previously unexplored lipids efficiently condense mRNA and mediate its in vivo delivery. Transfer of BMP-7 cmRNA lipoplexes to human mesenchymal stromal cells activates intracellular vesicle transport and cytoskeletal remodeling, and regulates extracellular matrix production and calcium-associated processes.