Omics · study · 2026
Bulk RNA-seq of SIT1 knockout and control T cells from human healthy donors
Listed in NCBI GEO
The transmembrane adaptor protein SIT1 is a negative regulator of T cell receptor (TCR) signaling, but its role in human immunity remains poorly characterized.
Description
We performed bulk RNA sequencing (RNA-seq) on T cells derived from human healthy donors. SIT1 knockout (KO) was generated in primary T cells, and transcriptomic profiles were compared between SIT1 KO and control cells.
This dataset enables the characterization of gene expression changes associated following SIT1 loss. Due to applicable data privacy regulations, identifiable information, including raw sequencing data from the participants, cannot be deposited in public repositories. Qualified researchers may request access to the data by submitting a formal application demonstrating compliance with the participants’ consent conditions.
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The consent signed by the study participants enables the use of data for study of rare immunological and hematological diseases. Requests should be directed to j.s.saarela@ncmbm.uio.no Processed gene expression data (read counts) and associated metadata are provided in this submission, providing a resource for studying SIT1-dependent transcriptional regulation in human T cells
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325729 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325729 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1441533 ncbi.nlm.nih.gov/bioproject/PRJNA1441533 ↗
project · from NCBI GEO
- PubMed 42128181 pubmed.ncbi.nlm.nih.gov/42128181 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE325729 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |