16S rRNA metabarcoding data and analysis code from throughfall and soil samples in a Neotropical forest (Ubatuba, São Paulo, Brazil)

The repository includes ASV tables, taxonomic classifications, and associated metadata. In addition to sequencing data, this repository provides R scripts used for downstream analyses, including diversity metrics (alpha and beta diversity), ordination analyses (PCoA), redundancy analysis (RDA), and structural equation modeling (piecewise SEM). Soil chemical variables (e.g., pH, organic matter, and nutrients) and microbial functional indicators—such as microbial biomass carbon, soil respiration, and β-glucosidase activity—were integrated into the analytical framework as well.

The data cannot be made publicly available due to intellectual property protection restrictions. For additional information, please contact the corresponding authors.