Omics · study · 2026
Feeder-Free Generation of Lymphatic Endothelial Cells from Human Induced Pluripotent Stem Cells
Listed in NCBI GEO
The lymphatic system is vital for fluid balance, lipid transport, and immune function, but studying human lymphatic vasculature has been limited by poor access to primary LECs.
Description
Here, we present an efficient, scalable, feeder-free protocol to differentiate human iPSCs into iLECs that closely resemble primary fetal LECs transcriptionally and phenotypically, enabling disease-relevant studies and precise genetic perturbations supported by marker validation and bulk RNA-seq comparisons to human fetal endothelial datasets.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE324nnn/GSE324673 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324673 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1436547 ncbi.nlm.nih.gov/bioproject/PRJNA1436547 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE324673 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |