Inclusion of maintenance energy improves the intracellular flux predictions of CHO

The pFBA predictions were compared to fluxes measured by 13C metabolic flux analysis (in 20 datasets from 6 publications), revealing that the predicted fluxes were largely underestimated. Computationally estimated maintenance energy (mATP) improved the predictions and was consistent with experimentally measured mATP.

The folder experimental_mATP contains data from continuous fermentations + scripts to calculate metabolite exchange rates, estimate mATP with pFBA and visualise the results. The folder pFBA_vs_13C contains 13C flux data from literature + scripts to run pFBA simulations (using the GSMM of CHO), compare pFBA-predicted and 13C fluxes, perform statistical analysis, computationally estimate mATP and visualise the results. More details can be found in read_me.txt files.