RNA-seq of mouse suprarenal aorta from VAMP8-knockout and wild-type mice with AngII or saline infusion

To investigate the role of VAMP8 in the vascular transcriptomic response to AngII infusion, we profiled gene expression in suprarenal aortae of VAMP8-knockout (Vamp8-/-) and wild-type C57BL/6 mice receiving 5-day continuous subcutaneous infusion of angiotensin II (AngII, 1,000 ng/kg/min) or saline via osmotic minipump.

Four experimental groups were generated: WT+Saline, WT+AngII, V8KO+Saline, and V8KO+AngII (n=4 per group). Differential expression analysis identified transcriptomic changes associated with AngII treatment, VAMP8 deficiency, and their interaction in the aortic wall.