Omics · study · 2026
HOXA3 regulates thymic epithelial cell differentiation from hESCs and supports T-cell differentiation through CXCL12
Listed in NCBI GEO
H9 human embryonic stem cells were differentiated into thymic epithelial cells (TECs) via a three-stage protocol.
Description
HOXA3 was knocked down during the 3PPE-to-TEC transition to investigate its role in TEC differentiation and function. RNA-seq was performed on HOXA3 knockdown and control TEC-like cells (3 biological replicates each).
HOXA3 knockdown disrupted epithelial differentiation and reduced TEC-associated gene expression, including K8, AIRE, and CD205, along with decreased FOXN1 protein. HOXA3-deficient TECs showed impaired support for T-cell generation from CD34+ progenitors. CXCL12 was identified as a downregulated gene and confirmed at mRNA and protein levels.
Read the rest (1 more)
Exogenous CXCL12 partially rescued the impaired T-cell-supporting function. This dataset provides insights into molecular regulation of human TEC differentiation.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344232 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344232 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1514989 ncbi.nlm.nih.gov/bioproject/PRJNA1514989 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE344232 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |