Omics · study · 2026
Tracing developmental and adult hematopoiesis with an endogenous zebrafish runx1- 2A-CreERT2 CRISPR knock-in
Listed in NCBI GEO
Zebrafish combines the power of genetics and unparalleled in vivo imaging to investigate the dynamics of vertebrate hematopoietic development.
Description
The conserved transcription factor Runx1 is essential for definitive hematopoiesis. We generated an endogenous zebrafish runx1-2A-creERT2 CRISPR knock-in for tamoxifen-regulated Runx1 lineage tracing and characterized its activity using recombinase-dependent fluorescence reporters, live imaging and flow cytometry.
Tamoxifen induction of Cre in the early embryo labeled all expected Runx1 lineages, including neuroectodermal olfactory placode and Rohan-Beard neurons, primitive hematopoietic blood cells, and emerging definitive hematopoietic stem and progenitor cells (HSPCs) in the dorsal aorta. Short windows of tamoxifen treatment allowed separation of Runx1 primitive from definitive hematopoiesis and visualization of HSPC colonization of the larval caudal hematopoietic tissue, thymus and kidney.
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Runx1 HSPCs labeled during development persisted into adulthood and contributed to hematopoietic precursors, myeloid, lymphoid, and peripheral blood lineages. Labeling of all blood lineages was also effective by tamoxifen treatment of 5-month-old adults. The runx1-2A-creERT2 knock-in line provides a novel genetic tool for inducible spatial and temporal analysis of Runx1 progenitor mechanisms in developmental and adult hematopoiesis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346550 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346550 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1525740 ncbi.nlm.nih.gov/bioproject/PRJNA1525740 ↗
project · from NCBI GEO
Topics
- Stated by source
- Danio rerio · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Imaging 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346550 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:imaging | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:7955 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |