Analysis of the correlation between the expression of the topoisomerase IIA(TOP2A) gene and the abundance of CD4+T cells and its corresponding immune mechanism in epithelial ovarian carcinoma.

Furthermore, the relationship between the TOP2A gene and CD4+T cells, cell subsets, various immune cells was investigated using TISIDB, ImmuCellAI, and TIMER databases. Additionally, the impact of CD4+T cell distribution on survival and prognosis of patients with EOC was assessed.Results The expression of TOP2A mRNA and protein was not significant in normal ovarian tissue and CD4+T cells, but was significantly high in EOC tissue, which was not conducive to the survival prognosis of patients.

The GO function of TOP2A co-expressed genes is mainly concentrated in the mitotic cell cycle G0 and early G1 division phase, PID-E2F pathway and transcriptional regulation of TP53, while KEGG is mainly concentrated in metabolic regulation, positive/negative feedback regulation of biological processes, stimulus response, cell cycle and cell growth and development. The expression of TOP2A gene was significantly correlated with the purity of tumor cells and the number of CD4+T cells and multiple immune cells.

A variety of immune cells have certain copy number variation in EOC, among which deep deletion is the most significant. However, only the number of CD8+T cells infiltrated by tumor microenvironment had a significant impact on survival and prognosis of EOC patients.Conclusion The expression of TOP2A mRNA in EOC tissues is positively correlated with the number of CD4+T cells, and the low distribution of tumor-infiltrating CD8+T cells is not conducive to the survival prognosis of EOC patients.