Influence of histone acetylation on chromatin 3D organization in Drosophila S2 cells [ChIP-seq]

The histone acetylation is known to disrupt both intra- and inter-nucleosomal interactions. However, its influence on the higher order chromatin organization has been studied to a lesser extent.

In this study, we changed global levels of histone acetylation in Drosophila S2 cells by treatment with the inhibitor of histone deacetylases trichostatin A (TSA) or the inhibitor of acetyltransferases curcumin and analyzed chromatin organization using chromatin immunoprecipitation sequencing (ChIP-seq) and high-throughput chromosome conformation capture (Hi-C) techniques. We found that, after TSA treatment, histone acetylation was notably increased in the active chromatin regions and decreased in the inactive regions, whereas, after curcumin treatment, histone acetylation has changed in the opposite directions.