Omics · study · 2026
Single-cell RNA-Seq analysis of rabbit filtering bleb conjunctiva
Listed in NCBI GEO
To investigate changes in fibroblasts in tissues after glaucoma filtration surgery (GFS) using mitomycin C (MMC), we performed scRNA-Seq on bleb-conjunctival tissues after GFS using MMC.
Description
We successfully sequenced 4349, 3683, 2588, and 2921 single cells from rabbit GFS tissue of control (without surgery), 3 hours, 3 days, and 14 days after surgery, respectively. An unbiased clustering of single cells projected onto a UMAP space identified 21 major clusters.
On days 3 and 14 after surgery, the number of identified fibroblasts was reduced to less than 30% compared to the control group, suggesting the effect of MMC on inducing cell death in fibroblasts. Thus, SASP-related factors such as CDKN1A, IL6, CXCL8, CCL2, and CCL7 were upregulated in fibroblasts after surgery.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307308 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307308 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1315186 ncbi.nlm.nih.gov/bioproject/PRJNA1315186 ↗
project · from NCBI GEO
- PubMed 42625400 pubmed.ncbi.nlm.nih.gov/42625400 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE307308 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |