Mortality Risk-Stratified Septic Serum Depresses Contractility and Mitochondrial Function in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes [SOMAscan]

Previously banked serum from children with septic shock (n=120) was stratified by Pediatric Sepsis Biomarker Risk Model (PERSEVERE) II mortality probability as low, intermediate, or high risk. We conducted aptamer-based proteomic analysis of septic serum to determine differentially expressed proteins in children with high compared to low mortality risk.

Sera from patients designated as PERSEVERE II high mortality risk demonstrated a global proteomic shift relative to those from patients with low mortality risk, and IL-6 and IL-8 were prominently differentially expressed proteins in children with high mortality probability. Downstream proteomic analyses for differential protein expression focused on comparing samples with HIGH and LOW PERSEVERE II risk, adjusting for site, age, and plate.