Omics · study · 2026
Semaglutide-induced Loss of Skeletal Muscle Mass is Blunted by Co-administration of Ketone Esters
Listed in NCBI GEO
Building on our recent work demonstrating that ketone ester therapy prevents semaglutide-induced cardiac mass loss in obese mice, we investigated whether ketone therapy could also preserve skeletal muscle mass during semaglutide treatment.
Description
To characterize the molecular changes associated with this treatment, we performed RNA-seq transcriptomic analysis of gastrocnemius muscle from obese mice treated with vehicle, semaglutide, or semaglutide + ketone ester.
Our analysis identified 5 dominant transcriptomic clusters enriched for pathways related to mitochondrial function, muscle contraction, translation, proteostasis, and extracellular matrix organization. Semaglutide increased expression of genes related to proteostasis and translation, whereas these changes were normalized with ketone ester co-treatment. In addition, semaglutide suppressed several mitochondrial genes, which were restored with ketone ester co-treatment.
Read the rest (1 more)
Furthermore, semaglutide reduced the expression of genes involved in the mitochondrial electron transport chain while increasing the expression of atrophy related genes, both of which were reversed by ketone ester co-treatment. Collectively, these findings suggest that ketone ester co-therapy mitigates semaglutide-induced transcriptomic alterations in skeletal muscle, providing mechanistic insight into how ketone therapy may help preserve muscle function during GLP-1based weight-loss therapy.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326952 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326952 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1447990 ncbi.nlm.nih.gov/bioproject/PRJNA1447990 ↗
project · from NCBI GEO
- PubMed 42262870 pubmed.ncbi.nlm.nih.gov/42262870 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Heart 65% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE326952 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |