Omics · study · 2026
Mesenchymal Stem Cells-derived Exosomes Inhibit Inflammatory Infiltration and Tissue Remodeling in Eosinophilic Chronic Rhinosinusitis
Listed in NCBI GEO
Exosomes generated from mesenchymal stem cells (MSCs-Exos) provide therapeutic promise for inflammatory diseases; however, their function in eosinophilic chronic rhinosinusitis (ECRS) is yet inadequately investigated.
Description
This research investigated the immunomodulatory and reparative properties of MSC-derived exosomes in a papain-induced mouse model of ECRS. MSCs-Exos were extracted and analyzed using transmission electron microscopy, NanoSight, and Western blotting.
In vivo, intranasal delivery of MSCs-Exos mitigated ECRS symptoms, diminishing sneeze, epithelial hyperplasia, mucus hypersecretion, and collagen deposition. Histopathological and immunohistochemical evaluations demonstrated reduced expression of FXIII-A, Clca1, and iNOS, along with elevated E-cadherin levels, signifying inhibition of epithelial-mesenchymal transition and excessive mucus formation. Flow cytometry revealed that MSCs-Exos rebalanced Th1/Th2/Th17 responses by increasing Th1 (IFN-γ⁺) cell populations and decreasing Th2 (IL-4⁺) and Th17 (IL-17⁺) populations.
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RNA sequencing and bioinformatic analysis revealed the downregulation of type 2 inflammatory genes (Ccl24, Ccl11) and epithelial remodeling indicators (Clca1, Mmp12), with the enrichment of pathways associated with immune regulation (IL-10 signaling, Treg differentiation) and tissue healing. KEGG pathway analysis further indicated the involvement of IL-17, JAK-STAT, and TNF signaling regulation in the therapeutic effects.
MSCs-Exos together alleviate ECRS by reestablishing immunological equilibrium, reducing inflammation, and preventing pathological remodeling, therefore establishing them as a viable cell-free treatment for chronic airway inflammatory conditions.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE302nnn/GSE302454 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE302454 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1290951 ncbi.nlm.nih.gov/bioproject/PRJNA1290951 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Microscopy 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE302454 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:microscopy | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |