Omics · study · 2026
RNA-seq analysis of human NSCLC cell lines treated with 9α,11α-dihydroxy-kaurenoic acid (AL-X)
Listed in NCBI GEO
Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide.
Description
In this study, we performed bulk RNA sequencing to investigate the transcriptomic effects of 9α,11α-dihydroxy-kaurenoic acid (9α,11αOH-KA, also referred to as AL-X) in human NSCLC cells. A549, HCC827, and H1975 cell lines were treated with 10 μg/mL 9α,11αOH-KA or vehicle (DMSO) for 48 hours.
Total RNA was extracted and subjected to paired-end sequencing using the Illumina platform. This dataset provides a comprehensive resource for understanding the molecular mechanisms underlying mitochondrial dysfunction and anti-cancer effects induced by 9α,11αOH-KA in NSCLC.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325732 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325732 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1441527 ncbi.nlm.nih.gov/bioproject/PRJNA1441527 ↗
project · from NCBI GEO
- PubMed 42191811 pubmed.ncbi.nlm.nih.gov/42191811 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE325732 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |