Omics · study · 2026
RNA‑seq transcriptomic analysis of HL‑1 cardiomyocytes under OGD/R injury with or without Phillyrin treatment
Listed in NCBI GEO
Ischemia‑reperfusion (I/R) injury is a key pathological process in myocardial infarction, for which effective therapies are still lacking.
Description
Phillyrin has been reported to exert cardioprotective effects, but its molecular mechanisms remain unclear. HL‑1 mouse cardiomyocytes were subjected to oxygen‑glucose deprivation/reoxygenation (OGD/R) to mimic I/R injury.
Cells were divided into two groups: OGD/R alone (IR) and OGD/R with Phillyrin treatment (IR+Phil). Total RNA was extracted from 3 biological replicates per group and sequenced using Illumina NovaSeq 6000. Transcriptomic profiling identified differentially expressed genes and pathways regulated by OGD/R and Phillyrin treatment.
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The processed data file contains raw read counts and FPKM values for all genes across all samples. This dataset provides a comprehensive transcriptomic resource to explore the molecular mechanisms of Phillyrin‑mediated cardioprotection against I/R injury.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333299 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333299 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1470647 ncbi.nlm.nih.gov/bioproject/PRJNA1470647 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Myocardial infarction 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE333299 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:myocardial-infarction | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |