Omics · study · 2026
Immunoprecipitation of m¹A methylated mRNA upon TRMT61B overexpression
Listed in NCBI GEO
The transcriptome-wide distribution of N¹-methyladenosine (m¹A) in mRNA remains contested, hindering a clear understanding of regulation of mRNA metabolism by m¹A. Additionally, the enzyme(s) that installs the majority of reported mRNA m¹A sites has yet to be identified.
Description
In this study, we characterized TRMT61B, an m¹A methyltransferase known to methylate mitochondrial RNAs, but whose exact sequence preferences have been underexplored.
We integrated in vivo overexpression of TRMT61B and in vitro methylation of a synthetic pool of diverse human RNA sequences to characterize the sequence preferences of TRMT61B target RNAs.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312403 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312403 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1373249 ncbi.nlm.nih.gov/bioproject/PRJNA1373249 ↗
project · from NCBI GEO
- PubMed 42059200 pubmed.ncbi.nlm.nih.gov/42059200 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE312403 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |